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Creating viral targets can weaken HIV vaccination, Emory scientists warn

November 21, 2019

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Lisa Newbern, 404-727-7709,

Vaccination against HIV can backfire if the vaccine induces the wrong kind of immune response. Scientists at Emory University's Yerkes National Primate Research Center have evidence -- gleaned from several nonhuman primate studies conducted with HIV's relative, SIV -- that creating too many soft targets can weaken vaccination that would otherwise provide protection against viral infection.

HIV targets and replicates in helper T cells, which aid in the body's antiviral immune response. The Emory researchers show that it is critical that a vaccine against HIV not create more safe havens for the virus than it does building defenses against it.

The Emory/Yerkes study results were published Nov. 20 in Science Translational Medicine.

The good news is scientists can detect early signs the immune system is going down the wrong track, says senior author Rama Rao Amara, PhD. Amara is a Yerkes and Emory Vaccine Center researcher, and co-director of the Emory Consortium for Innovative AIDS Research in Nonhuman Primates.

The problem comes when vaccination generates too many of a particular type of helper immune cell, Th1 cells. These cells travel to mucosal tissues, such as the rectum, cervix and vagina, where HIV/SIV first enters the body in the majority of infections.

The Th1 cells are like first-responders to a zombie attack. They fight the virus initially, but then they get taken over. What's needed instead are Tfh cells, which stay in the lymph nodes and help the immune system make antibodies against the virus, Amara says.

"We're not saying Th1 cells are bad," he says. "But if you have too many, they take away from effective vaccine protection."

"It's a matter of stimulating just the right amount of immune help for a strong immune response, but not so much that it increases susceptibility to the virus," says co-author Eric Hunter, PhD, co-director of the Consortium and co-director of Emory's Center for AIDS Research.

Immunologists suspected it was possible for vaccination against HIV to be counterproductive. The HVTN 502/STEP clinical trial (2004-2007) appears to have run aground for this reason. But the field has learned a great deal since then.

Looking ahead, researchers in the HIV vaccine field are feeling optimistic, and large-scale clinical trials are under way, following on the success of the RV144 trial in Thailand a decade ago.

"We need to build information about how adjuvants steer the immune response into future clinical studies," Amara says. "Vaccine efficacy depends not only on the magnitude of the immune response but also on its quality."

Adjuvants are vaccine components that enhance the immune response, and some HIV vaccine studies now being conducted combine adjuvants with synthetic copies of parts of HIV.

For the Science Translational Medicine paper, the Emory researchers gathered information from four studies on macaques immunized against SIV or the hybrid SHIV virus. Depending on the study, vaccinated monkeys were challenged intravaginally or intrarectally -- the two most common routes of infection in humans.

As an example, for the intravaginal SHIV study, vaccinated animals with a high level of antiviral Th1 cells showed no delay in SHIV infection compared with unvaccinated animals. However, those with a low level of Th1s did have some level of protection against SHIV -- two out of seven stayed uninfected after eight challenges. A similar effect was seen with SIV and intrarectal, when significant protection was observed, but only for Th1-low animals.

The Th1 cells identified as compromising protection tended to persist in the mucosal tissues more than in the blood, where they were sometimes hard to detect. Amara says scientists studying candidate HIV vaccines in humans should examine whether they also create too many Th1 target cells. That could be cumbersome if researchers have to take mucosal tissue samples from study participants.

"A few months after vaccination, we saw it's difficult to see the Th1s in the blood, compared with the mucosa," Amara says. "But we could see a gene expression signature in the blood, one week after priming, which gave us a prediction of what will be in the mucosal tissues later."
This information could help immunologists design vaccines that provide more reliable protection against HIV, he says.

The co-first authors of the paper are postdoctoral fellow Venkateswarlu Chamcha, PhD, now at Merck; research associate Pradeep Reddy, PhD, now at Pfizer; and research associate Sunil Kannanganat, PhD, now at Houston's Methodist Hospital Research Institute.

Emory/Yerkes co-authors include Mehul Suthar, PhD; Guido Silvestri, MD; and Cynthia Derdeyn, PhD. Additional contributions came from Emory bioinformatics expert Tianwei Yu; Michael Gale, PhD, from University of Washington; and Francois Villinger, DVM, PhD, now at New Iberia Research Center in Louisiana.

The research was supported by the National Institute of Allergy and Infectious Diseases (PO1AI088575, U19AI096187, U19AI096187, UM1AI124436), the Emory Center for AIDS Research (P30AI050409) and the NIH Director's Office of Research Infrastructure Programs (Primate centers: P51OD011132).

Dedicated to discovering causes, preventions, treatments and cures, the Yerkes National Primate Research Center (NPRC), part of Emory University’s Robert W. Woodruff Health Sciences Center, is fighting diseases and improving human health and lives worldwide. The center, one of only seven NPRCs the National Institutes of Health (NIH) funds, is supported by more than $73 million in research funding (all sources, fiscal year 2019). Yerkes researchers are making landmark discoveries in microbiology and immunology; neurologic diseases; neuropharmacology; behavioral, cognitive, and developmental neuroscience; and psychiatric disorders. Since 1984, the center has been fully accredited by the AAALAC International, regarded as the gold seal of approval for laboratory animal care. For more information about Yerkes and the seven NPRCs, visit and follow us at @NPRCnews.



The Robert W. Woodruff Health Sciences Center of Emory University is an academic health science and service center focused on missions of teaching, research, health care and public service. Its components include the Emory University School of Medicine, Nell Hodgson Woodruff School of Nursing, and Rollins School of Public Health; Yerkes National Primate Research Center; Winship Cancer Institute of Emory University; and Emory Healthcare, the largest, most comprehensive health system in Georgia. Emory Healthcare includes: The Emory Clinic, Emory-Children's Center, Emory University Hospital, Emory University Hospital Midtown, Wesley Woods Center, and Emory University Orthopaedics & Spine Hospital. The Woodruff Health Sciences Center has a $2.5 billion budget, 17,600 employees, 2,500 full-time and 1,500 affiliated faculty, 4,700 students and trainees, and a $5.7 billion economic impact on metro Atlanta.

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